F6057

Fluoroshield^™ with DAPI

liquid

• Code UNSPSC : 12171500
• Nomenclature NACRES : NA.47

Propriétés

• Product Name: Fluoroshield^™ with DAPI, liquid
• Description: histology mounting medium
• Niveau de qualité: 200
• Forme: liquid
• Composition: Sodium Azide, ≤1% (also contains other ingredients); Tris-HCl
• Technique(s): immunofluorescence: suitable
• Plage de pH: 7.9-8.3
• Solubilité: water: soluble
• Fluorescence: λ_ex 360 nm; λ_em 460 nm
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-8°C

Description

Description générale

Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Application

Fluoroshield^™ with DAPI has been used:

• as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
• as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
• as mounting solution for immunostained heart sections for nuclei detection

Informations légales

FluoroShield is a trademark of ImmunoBioScience Corp.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable