F6432

FLT3 (571-993), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonyme(s) : CD135, FLK2, STK1
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Niveau de qualité: 200
• Gamme de produits: PRECISIO^® Kinase
• Essai: ≥70% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• Activité spécifique: 60-81 nmol/min·mg
• Poids mol.: ~73 kDa
• Numéro d'accès UniProt: P36888
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... FLT3(2322)

Description

Description générale

FLT3 (fms-related tyrosine kinase 3) is a tyrosine kinase receptor and the gene is localized to human chromosome 13q12. The encoded protein is composed of 993 amino acids, and consists of five immunoglobulin (Ig)-like domains in its exoplasmic region. It is also composed of a transmembrane region, a juxtamembrane domain, and two tyrosine kinase domains in its intracellular domain. The tyrosine kinase domains are linked via a kinase-insert domain.

Actions biochimiques/physiologiques

FLT3 (fms-related tyrosine kinase 3), upon interaction with its ligand, undergoes conformational changes to form homodimers. This results in phosphorylation and activation of downstream signaling pathways including phosphatidylinositol 3-kinase (PI3K/AKT), mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) and signal transducer and activator of transcription 5 (STAT5) pathways. Internal tandem duplication (ITD) mutation or a point mutation in juxtamembrane domain-coding region or within the activation loop domain, respectively, of this gene lead to its constitutive action. FLT3/ITD mutation is associated with later stages of acute myeloid leukemia (AML) pathogenesis. These patients have poor prognosis.

Forme physique

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Informations légales

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317,H319,H412
• Conseils de prudence: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable