FNCAS9PROT
FnCas9 Protein
from Francisella novicida, recombinant, expressed in E. coli, 1X NLS
Synonyme(s) :
Francisella Novicida Cas9
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.51
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Essai
≥95% (SDS-PAGE)
Forme
lyophilized powder
Conditionnement
pkg of 1 kit (4 components)
Application(s)
CRISPR
Conditions d'expédition
wet ice
Température de stockage
−20°C
Catégories apparentées
Description générale
The purified FnCas9 protein (~190 kDa) is derived from the type II CRISPR/Cas system from the bacterium Francisella novicida and has been engineered to function using three molecular components: the FnCas9 protein, a crRNA of user-defined sequence, and a tracrRNA of constant sequence. The FnCas9 endonuclease can be programmed by changing the crRNA sequence to create a site-specific double-strand break (DSB) in plasmids or purified genomic DNA.
Application
Functional Genomics/Target Validation/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology
Caractéristiques et avantages
- Suitable for plasmid or large genomic DNA fragment cloning
- Results in 4-bp 5′ overhangs.
- Cuts purified plasmid DNA more efficiently than SpCas9
Conditionnement
pkg of 50 μg (≥ 300 pmol)
pkg of 250 μg (≥ 1500 pmol)
pkg of 250 μg (≥ 1500 pmol)
Composants
Each kit consists of:
- one vial of FnCas9 recombinant protein
- one vial containing 1 mL of 1× Dilution buffer
- one vial containing 1 mL of Nuclease free water with glycerol
- one vial containing 1 mL of 10× Digestion Buffer
Principe
Conventional restriction enzymes such as EcoRI and HindIII can only cut specific short (6 bp) DNA sequences and are therefore limited to cloning small DNA fragments (typically ~4 kb). CRISPR-based endonculeases can cut user defined sequences of a longer length (~20 bp) making them more flexible and applicable to larger DNA fragments such as multigene bacterial operons or mammalian genomic DNA with native promoter, exon, and intron components.
Reconstitution
Lyophilized FnCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.
Autres remarques
Click here to order gRNA
Enter the 20bp genomic DNA target sequence (5′ to 3′) plus the adjacent 3′ PAM site. Under special instructions, indicate "FnCas9 crRNA"
Click here to order FnCas9 tracrRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Enter the 20bp genomic DNA target sequence (5′ to 3′) plus the adjacent 3′ PAM site. Under special instructions, indicate "FnCas9 crRNA"
Click here to order FnCas9 tracrRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Informations légales
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Composants de kit seuls
Réf. du produit
Description
- FnCas9-NLS from Francisella novicida, expressed in Escherichia coli
- Dilution buffer for Cas9 proteins
- Reconstitution solution for Cas9 proteins
- Digestion buffer 10× for FnCas9
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2
Code de la classe de stockage
11 - Combustible Solids
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