G0282

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : GM-CSF
• Numéro MDL: MFCD00241918
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Product Name: Granulocyte-Macrophage Colony-Stimulating Factor from mouse, GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Essai: ≥97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: ≤0.2 ng/mL EC₅₀ (corresponds to ≥5 × 10⁶ units/mg)
• Qualité: endotoxin tested
• Poids mol.: ~15 kDa (125 amino acids including N-terminal methionine)
• Conditionnement: pkg of 5 and 50 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/mg
• Couleur: white
• Solubilité: water: soluble, clear, colorless
• Numéro d'accès UniProt: P01587
• Température de stockage: −20°C
• Informations sur le gène: mouse ... CSF2(12981), Csf2(12981)

Description

Actions biochimiques/physiologiques

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Forme physique

Lyophilized from 10 mM acetic acid plus 250 μg BSA.

Remarque sur l'analyse

The EC₅₀ activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)