G1163

O-Glycosidase from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

• Synonyme(s) : Endo-α-N-acetylgalactosaminidase, O-Glycanase
• CAS Number: 9032-92-2
• Numéro CE : 3.2.1.97 (BRENDA, IUBMB)
• Numéro CE : 232-888-2
• Numéro MDL: MFCD00131003
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Conjugué: (O-linked)
• Forme: buffered aqueous solution
• Poids mol.: 180 kDa
• Concentration: ≥800 units/mL
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Actions biochimiques/physiologiques

Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..

Conditionnement

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄ pH 5.0.

Définition de l'unité

One unit will hydrolyze 1 μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→ΟC₆H₄NO₂) per min at 37 °C at pH 6.5.

Forme physique

Solution in 50 mM sodium phosphate, pH 7.5

Remarque sur l'analyse

Screened for presence of: β-galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases. See Certificate of Analysis for lot specific information.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)