H1404

Hepatocyte Growth Factor human

>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture

• Synonyme(s) : HGF, Hepatopoietin A, Scatter Factor
• Numéro CE : 214-275-1
• Numéro MDL: MFCD00217422
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Product Name: Hepatocyte Growth Factor human, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture
• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in Baculovirus infected High-5 cells
• Essai: >95% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 1.0-20.0 ng/mL
• Qualité: endotoxin tested
• Poids mol.: ~80 kDa
• Conditionnement: pkg of 5x5 μg; pkg of 5 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <1.0 EU/μg
• Numéro d'accès UniProt: P14210
• Température de stockage: −20°C
• Informations sur le gène: human ... HGF(3082)

Description

Actions biochimiques/physiologiques

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Autres remarques

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH₂PO₄ and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

Notes préparatoires

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

Remarque sur l'analyse

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves