H4791

BMP2, human

BMP-2, recombinant, expressed in HEK 293 cells, HumanKine, suitable for cell culture

• Synonyme(s) : Bone Morphogenetic Protein 2 human, BMP-2
• Numéro MDL: MFCD03097877
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Product Name: Bone Morphogenetic Protein 2 human, BMP-2, recombinant, expressed in HEK 293 cells, HumanKine, suitable for cell culture
• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in HEK 293 cells
• Forme: lyophilized powder
• Puissance: ≤60 ng/mL EC₅₀
• Qualité: endotoxin tested
• Poids mol.: dimer 30-38 kDa (glycosylated)
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg Endotoxin level
• Numéro d'accès UniProt: P12643
• Température de stockage: −20°C
• Informations sur le gène: human ... BMP2(650)

Description

Description générale

Bone Morphogenetic Protein 2 is a member of the TGF-β superfamily of cytokines that affect bone and cartilage formation. It is important for skeletal development during embryogenesis. BMP-2 induces chondrocyte formation, osteoblast differentiation, and is involved in embryo dorsal-ventral patterning and organogenesis.

Actions biochimiques/physiologiques

It has been reported that Bone Morphogenetic Protein 2 (BMP-2) inhibits estradiol induced proliferation of human breast cancer cells. BMP-2 signaling mediates apoptosis by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6. Cellular responses to BMP-2 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors, which play significant roles in BMP binding and signaling. One BMP type II receptor and two BMP type I receptors have been identified. Both BMP type I receptors bind BMP-2 with high-affinity in the absence of BMP receptor type II.

Forme physique

Lyophilized from a 0.2 μm filtered solution of 2x PBS + 6% Ethanol.

Notes préparatoires

This Bone Morphogenetic Protein 2 (BMP-2) is produced from a DNA sequence encoding the human BMP-2 protein, expressed in HEK 293 cells. It is a glycosylated homodimer linked by a single disulfide bond with an apparent molecular mass of 30-38 kDa.

Remarque sur l'analyse

The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (mouse chondrogenic cell line).

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable