H6666

Facteur de croissance des kératinocytes human

HumanKine^®, >95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonyme(s) : FGF-7, KGF
• CAS Number: 148348-15-6
• Numéro MDL: MFCD00212245
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Product Name: Facteur de croissance des kératinocytes human, KGF, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Essai: >95% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 4-20 ng/mL EC₅₀
• Qualité: endotoxin tested
• Poids mol.: dimer 17-30 kDa (glycosylated)
• Conditionnement: pkg of 10 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <1 EU/μg
• Numéro d'accès UniProt: P21781
• Température de stockage: −20°C
• Informations sur le gène: human ... FGF7(2252)

Description

Description générale

Keratinocyte Growth Factor (KGF) belongs to the fibroblast growth factor 7 (FGF-7) family. It is produced by the mesenchymal cells, γδ T cells, and fibroblasts in several organs. The KGF gene is located on the human chromosome at 15q21.2.

Application

Keratinocyte Growth Factor (KGF) human has been used as a component of the keratinocyte differentiation medium.

Actions biochimiques/physiologiques

Keratinocyte Growth Factor (KGF) exerts paracrine effects on mesenchymal cells. It exhibits cryoprotective effects on epithelial cells of the lungs, gastrointestinal tract, and the lungs. KGF participates in the activation of IIIb isoform of the fibroblast growth factor (FGF)-receptor 2 (FGFR2-IIIb) present on keratinocytes. This activation further favors proliferation, migration, and survival of keratinocytes.

Forme physique

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Notes préparatoires

HumanKine Keratinocyte Growth Factor (KGF) is expressed as glycosylated 17 and 30 kDa monomers in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Remarque sur l'analyse

The activity was determined by the dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line).

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable