H7039

3-Hydroxy-3-methylglutaryl-CoA reductase human

solution

• Synonyme(s) : HMGCoA Reductase, HMGR
• Numéro CE : 1.1.1.34 (BRENDA, IUBMB)
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Essai: ≥90% (SDS-PAGE)
• Niveau de qualité: 200
• Forme: solution
• Activité spécifique: 2-8 units/mg protein
• Poids mol.: ~76 kDa (for the fusion protein)
• Numéro d'accès UniProt: P04035
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... HMGCR(3156)

Description

Application

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:

• in anti-HMGCR enzyme linked immunosorbent assay (ELISA)
• as an antigen to measure anti-HMGCR antibodies by blot analysis
• in HMG-CoA reductase activity assays
• as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot

Actions biochimiques/physiologiques

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).

Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

Propriétés physiques

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Définition de l'unité

One unit will convert 1.0 μmole of NADPH to NADP⁺ per minute at 37 °C.

Forme physique

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable