HT10132

Bouin′s solution

histological fixative

• Synonyme(s) : Bouin’s fluid
• Numéro MDL: MFCD00146169
• Code UNSPSC : 12171500
• Nomenclature NACRES : NA.47

Propriétés

• Niveau de qualité: 200
• Durée de conservation: Expiry date on the label
• Composition: acetic acid, 5% ; formaldehyde, 9% ; picric acid, 0.9%
• Couleur: yellow
• Application(s): hematology; histology
• Température de stockage: room temp

Description

Description générale

Bouin′s solution is a noncoagulant yellow stain fixative that consists of saturated picric acid, formaldehyde, and acetic acid.

Application

Bouin′s solution is an excellent fixative for preserving soft and delicate structures. It is used as a mordant in various trichrome procedures.
Additionally, it has been used for the following purposes-

• staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
• fixation of rat ovaries for the observing follicle cells using microscopy
• fixation of gonads of crab for morphological and histological analysis

Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Actions biochimiques/physiologiques

Bouin′s solution causes shrinkage of tissues when used as a preservative due to its picric acid component. It preserves morphological and nuclear information in tissues upon fixation.

Informations sur la sécurité

• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter