I1146

R³ IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : Human IGF-1, IGF-1 Protein, Recombinant IGF-1
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Essai: ≥85% (HPLC)
• Forme: lyophilized powder
• Puissance: ≤10 ng/mL ED₅₀
• Qualité: endotoxin tested
• Poids mol.: 7,676 Da
• Conditionnement: pkg of 0.1 mg
• Technique(s): cell culture | mammalian: suitable
• Numéro d'accès UniProt: P05019
• Température de stockage: 2-8°C
• Informations sur le gène: human ... IGF1(3479)

Description

Application

R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

Actions biochimiques/physiologiques

Proliferation of chinese hamster ovary CHO cells.

Autres remarques

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Notes préparatoires

Prepared from 0.1 M acetic acid

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)