I1645

Interleukin-8 human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : IL-8
• Numéro MDL: MFCD00131404
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Essai: ≥98% (SDS-PAGE and HPLC)
• Forme: lyophilized powder
• Poids mol.: 8.4 kDa
• Conditionnement: pkg of 10 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: P10145
• Température de stockage: −20°C
• Informations sur le gène: human ... IL8(3576)

Description

Application

Interleukin-8 (IL-8) human has ben used to:

• measure neutrophil chemotoxis
• study the uptake of apoptotic cells by meningothelial cells
• study the effect of IL-8 on expression and secretion of urocortin-1 by human umbilical vein endothelial cells

Actions biochimiques/physiologiques

Interleukin-8 (IL-8) was formerly called monocyte-derived neutrophil chemotactic factor. It belongs to the chemokine α or C-X-C family. Like other members of this family, the mature form of IL-8 has four cysteine residues and the first two cysteine residues are separated by glutamine. IL-8 shows chemotactic activity in vitro for T cells, neutrophils and basophils. Mutation in IL-8 can increase risk to acute pancreatitis.

Interleukin-8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the chemokine α or C-X-C family. The mature form of IL-8 has 4 cysteine residues, as do the other members of the chemokine family and the first two cysteine residues are separated by glutamine. Mature human IL-8 consists of 72 amino acids with a molecular mass of 8.4 kDa. IL-8 exhibits chemotactic activity in vitro for T cells, basophils, as measured by enzymes including myeloperoxidase, α-mannosidase and β-glucuronidase. IL-8 is an angiogenic factor.

Forme physique

Lyophilized from a 0.2 μm filtered solution with no additives.

Remarque sur l'analyse

The biological activity is determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 10.0-100.0 ng/ml.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)