I7276

Anti-Insulin-Like Growth Factor-II antibody produced in goat

affinity isolated antibody, lyophilized powder

• Synonyme(s) : Anti-IGF-II
• Numéro MDL: MFCD00162567
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: goat
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: lyophilized powder
• Espèces réactives: human
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5-15 μg/mL using human Placenta; neutralization: 0.2-0.8 μg/mL; western blot: suitable
• Numéro d'accès UniProt: P01344
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... IGF2(3481)

Description

Description générale

Insulin-like growth factors -I and -II are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -II (IGF-II) is widely expressed during embryonic stages and regulates the placental development. IGF-II acts via specific receptor, IGF-IIR, the binding does not result in signal transduction, but is a mechanism to restrict the bioavailability of IGF-II. IGF-II also binds to IGF-IR which results in the activation of MAPK, Raf/Ras and PI3K cascades. The effects of IGF-II signalling are important in growth of blood vessels (angiogenesis) gene transcription, proliferation and transformation. Irregular IGF-II signaling has been implicated in many cancers including lung cancer, colo-rectal cancer, cervical cancer, testicular cancer, rhabdosarcoma, melanoma and hepatoblastoma
Anti-IGF-II recognizes human insulin-like growth factor II. It does not react with human IGF-I.

Spécificité

The antibody will neutralize the biological activity of recombinant human IGF-II. It does not neutralize the biological activity of recombinant human IGF-I.

Immunogène

recombinant human IGF-II

Application

Anti- IGF-II antibody may be used for neutralization reactions, the ND50 of the antibody is 0.2-0.8 μg/ml. This antibody was used to neutralize IGF-II in glioblastoma cell lines. For immunohistochemistry a working antibody concentration of 5-15 μg/ml may be used for formalin-fixed, paraffin-embedded human placenta sections. The antibody is suitable for immunoblotting.

Forme physique

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, with 5% trehalose

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)