L2005

Lactoperoxidase from bovine milk

lyophilized powder (essentially salt-free), ≥200 units/mg protein

• Synonyme(s) : Donor:hydrogen peroxide oxidoreductase, Peroxidase
• CAS Number: 9003-99-0
• Numéro CE : 1.11.1.7 (BRENDA, IUBMB)
• Numéro CE : 232-668-6
• Numéro MDL: MFCD00131484
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine milk
• Niveau de qualité: 200
• Forme: lyophilized powder (essentially salt-free)
• Activité spécifique: ≥200 units/mg protein
• Rapport des absorbances: A_{412/280 nm} 0.7-0.9
• Numéro d'accès UniProt: P80025
• Température de stockage: −20°C
• Chaîne SMILES: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• Clé InChI: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Description

Description générale

Lactoperoxidase is a major enzyme present in bovine milk and belongs to the peroxidase family. It is present in both plants and animals. The enzyme structure comprises a single polypeptide chain made up of 612 amino acid residues.

Application

Lactoperoxidase from bovine milk has been used as a standard for milk lactoperoxidase to study the role of H₂O₂ in the inhibition of Staphylococcus aureus growth by Lactococcus garvieae in the presence of lactoperoxidase in raw milk. It has also been used in lactoperoxidase-mediated iodination to prepare radiolabeled peptides.

Actions biochimiques/physiologiques

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

Lactoperoxidase contributes to the antimicrobial system of milk by inactivating a wide range of micro-organisms. This lactoperoxidase-mediated antimicrobial system is also identified in human secretions such as tear-fluid, saliva, and milk. Lactoperoxidase catalyzes the oxidation of molecules by releasing H2O2. The product exhibits antimicrobial activity.

Définition de l'unité

One unit will oxidize 1.0 μmole of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 5.5 at 25 °C.

Remarque sur l'analyse

Protein determined by Lowry method.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)