L2011

D-Lactic Dehydrogenase from Lactobacillus leichmannii

ammonium sulfate suspension, ≥250 units/mg protein (biuret)

• Synonyme(s) : Lactate, (R)-Lactate:NAD⁺ oxidoreductase, D−LDH
• CAS Number: 9028-36-8
• Numéro CE : 1.1.1.28 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130907
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: ammonium sulfate suspension
• Niveau de qualité: 200
• Activité spécifique: ≥250 units/mg protein (biuret)
• Activité étrangère: Malic dehydrogenase <0.5% of base activity
• Température de stockage: 2-8°C

Description

Description générale

Research area: Cell Signalling D-lactate dehydrogenase belongs to the D-isomer-specific 2-hydroxyacid dehydrogenase family. Lactate dehydrogenase (LD) is a tetramer consisting of two (H and M) subunits. It has five isozymes (LD1 to LD5) and this composition varies in different tissues. LD1 is abundant in the kidney, heart, and erythrocytes. LD5 is found in the liver and skeletal muscles. LD is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family.

Actions biochimiques/physiologiques

Lactic dehydrogenase (LDH)plays an important role in the glycolytic pathway where it converts pyruvate tolactate by using NAD⁺ as a co-factor. It is considered animportant molecule that can be used in cancer therapy as it acts as aglycolytic inhibitor. Inhibition of LDH is associated with blocking of aerobicglycolysis in tumour cells.

Définition de l'unité

One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.

Forme physique

Suspension in 3.2 M (NH₄)₂SO₄, pH 6.0

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)