L4516

Lipopolysaccharides from Escherichia coli O127:B8

BioXtra, suitable for cell culture, γ-irradiated

• Synonyme(s) : LPS
• CAS Number: 93572-42-0
• Numéro CE : 297-473-0
• Numéro MDL: MFCD00212998
• Code UNSPSC : 12352211
• Nomenclature NACRES : NA.75

Propriétés

• Source biologique: Escherichia coli (O127:B8)
• Niveau de qualité: 200
• Stérilité: γ-irradiated
• Gamme de produits: BioXtra
• Forme: lyophilized powder
• Produit purifié par: gel-filtration chromatography
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <1% Protein (Lowry)
• Solubilité: H₂O: 5 mg/mL, slightly hazy
• Température de stockage: 2-8°C

Description

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Lipopolysaccharides from Escherichia coli 0127:B8 has been used:

• to study the LPS-induced macrophage survival in mice
• to elicit the secretion of cytokines by cattle PBMC
• to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment
• to perform autophagy assay in BV2 and BV2-LC3 cells
• for stimulation of human monocytes, macrophages and mouse microglia cells
• to induce pentraxin-3 expression in 293T cells

Actions biochimiques/physiologiques

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4). Being an immunogenic antigen, it enhances immune responses to soluble antigens.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informations sur la sécurité

• Pictogrammes: GHS06
• Mention d'avertissement: Danger
• Mentions de danger: H300
• Conseils de prudence: P264 - P270 - P301 + P310 - P405 - P501
• Classification des risques: Acute Tox. 2 Oral
• Code de la classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable