L7381

Lectin from Arachis hypogaea (peanut)

FITC conjugate, lyophilized powder

• Synonyme(s) : PNA, Peanut agglutinin
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Conjugué: FITC conjugate
• Niveau de qualité: 200
• Forme: lyophilized powder
• Composition: Protein, ~10% Lowry
• Ampleur du marquage: 4-8 mol FITC per mol protein
• Température de stockage: −20°C

Description

Description générale

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Lectin from Arachis hypogaea (peanut) has been used:

• to determine the acrosomal status (presence or absence of acrosomal matrix corresponding to intact or acrosome-reacted spermatozoa) on viable sperm cells
• to probe cryosections of mediastinal lymph nodes (mLNs) with fluorochrome-labeled PNA and anti-B220 to detect germinal centers (GCs)
• to visualize the cone outer segments of pre-treated retinal sections

Actions biochimiques/physiologiques

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.

PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.

Forme physique

Contains phosphate buffer salts and NaCl

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)