L8402

Lysozyme from human neutrophils

≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

• Synonyme(s) : Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 9001-63-2
• Numéro CE : 3.2.1.17 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131557
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: human neutrophils
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Essai: ≥95% (SDS-PAGE)
• Forme: lyophilized powder
• Activité spécifique: ≥100,000 units/mg protein (E1%/280)
• Technique(s): cell based assay: suitable
• Adéquation: suitable for cell lysis
• Numéro d'accès UniProt: P61626
• Application(s): cell analysis
• Température de stockage: −20°C
• Chaîne SMILES: N1([C@@H](CCC1)C(=O)N[C@@H](CCC(=O)N)C(=O)NCC(=O)N[C@@H]([C@H](CC)C)C(=O)N[C@@H](CCCN\C(=N\[H])\N)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc4c5c([nH]c4)cccc5)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc2c3c([nH]c2)cccc3)C(=O)N[C@@H](CCCN\C(=N\[H])\N)C(=O)N[C@@H]
• InChI: 1S/C99H159N37O23/c1-11-50(8)77(132-72(139)46-120-81(145)63(32-33-70(101)137)124-88(152)69-31-21-39-136(69)93(157)68(43-73(140)141)131-84(148)62(29-19-37-116-98(109)110)125-90(154)75(48(4)5)135-91(155)76(49(6)7)133-80(144)57(100)24-16-34-113-95(103)104)92(156)126-60(27-17-35-114-96(105)106)82(146)121-51(9)78(142)129-66(41-54-45-119-59-26-15-13-23-56(54)59)87(151)134-74(47(2)3)89(153)122-52(10)79(143)128-65(40-53-44-118-58-25-14-12-22-55(53)58)85(149)123-61(28-18-36-115-97(107)108)83(147)130-67(42-71(102)138)86(150)127-64(94(158)159)30-20-38-117-99(111)112/h12-15,22-23,25-26,44-45,47-52,57,60-69,74-77,118-119H,11,16-21,24,27-43,46,100H2,1-10H3,(H2,101,137)(H2,102,138)(H,120,145)(H,121,146)(H,122,153)(H,123,149)(H,124,152)(H,125,154)(H,126,156)(H,127,150)(H,128,143)(H,129,142)(H,130,147)(H,131,148)(H,132,139)(H,133,144)(H,134,151)(H,135,155)(H,140,141)(H,158,159)(H4,103,104,113)(H4,105,106,114)(H4,107,108,115)(H4,109,110,116)(H4,111,112,117)/t50-,51-,52-,57-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,74-,75-,76-,77-/m0/s1
• Clé InChI: ZJCXKXFAOCLSRV-RYLVIXIQSA-N
• Informations sur le gène: human ... LYZ(4069)

Description

Description générale

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

Application

Lysozyme from human neutrophils has been used in the checkerboard method for establishing a synergy between peptidomimetic 1 and immune effector molecules against E. coli.

Actions biochimiques/physiologiques

Lysozymes participates in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

Définition de l'unité

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Forme physique

Lyophilized from 50 mM sodium acetate, pH 6.0, with 100 mM NaCl

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable