M0643

Milieu essentiel minimum d′Eagle

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

• Synonyme(s) : MEM
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.75

Propriétés

• Niveau de qualité: 500
• Forme: powder
• Technique(s): cell culture | mammalian: suitable
• Composants: HEPES: no; Earle’s salts (5% CO₂): yes; NaHCO₃: no; phenol red: yes; L-glutamine: yes; sodium pyruvate: no
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids. It is widely used as a media formulation for many cell culture studies. It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells, and endometrial adenocarcinoma Ishikawa cells.

Quantité

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable