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MDQ1

Sigma-Aldrich

Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

Synonyme(s) :

DNA Methylation Quantification, DNA methylation shift

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.54

Description générale

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Application

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Caractéristiques et avantages

  • ELISA based format
  • Procedure completes in 4 easy to follow steps
  • All reagents supplied including control methylated DNA
  • Detection limit is 5 ng of fully methylated DNA
  • Input DNA may be as low as 10-200 ng
  • Procedure completes in 3.5 hours
  • 96 well format allows option of studying single samples or high-throughput studies
  • Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
  • May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Informations légales

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • M8570Methylated Control DNA, 50 ng/μLFDS

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Pictogrammes

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GHS05,GHS07

Mention d'avertissement

Warning

Mentions de danger

H290,H317,H412

Classification des risques

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

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