MTOX1013

HepaRG^™ Cells AHR (-/-)

human female liver (hepatocarcinoma and hepatitis C tumor)

• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.81

Propriétés

• Product Name: HepaRG^™ Cells AHR (-/-), one vial, from human female liver(hepatocarcinoma and hepatitis C tumor)
• Source biologique: human female liver (hepatocarcinoma and hepatitis C tumor)
• Niveau de qualité: 200
• Forme: liquid
• Température de stockage: −196°C
• Informations sur le gène: human ... AHR(196)

Description

Description générale

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG AHR knockout cells express the major xenobiotic sensors (PXR and CAR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Application

See technical bulletin for detailed protocols

Caractéristiques et avantages

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity. - Sigma′s HepaRG AHR Knockout (KO) allows conclusive identification of CYP1A1 and CYP1B1 induction through AHR activation.

Zinc finger nucleases (ZFN) mediated Knockout of AHR (AHR) gene.

• The frame-shift mutation of AHR gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.

Qualité

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Informations légales

Exhibit 1: ADME/Tox cell lines license
Exhibit 2: HepaRG limited use license

HepaRG is a trademark of BioPredic International company

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable