N1408

Nerve Growth Factor β Human

>97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• Synonyme(s) : HSAN5, NGF-β human, NGFB, hNGFβ
• Numéro MDL: MFCD00164982
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Product Name: NGF-β human, from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in NSO cells
• Essai: >97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 0.15-3.0 ng/mL ED₅₀/EC₅₀
• Qualité: endotoxin tested
• Conditionnement: pkg of 5X0.1 mg; pkg of 0.1 mg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <1 EU/μg
• Solubilité: water: soluble 0.1 g/L at 20 °C
• Numéro d'accès UniProt: P01138
• Température de stockage: −20°C
• Informations sur le gène: human ... NGF(4803)

Description

Description générale

The nerve growth factor β (NGFB) gene is mapped to human chromosome 1p13.2.

Application

NGF-β human has been used:

• in protein pin arrays
• to induce U87-MG (glioblastoma cell line) cell proliferation
• in drug release kinetics

Actions biochimiques/physiologiques

Nerve growth factor β (NGFB) acts as a key mediator in neuronal and non-neuronal development, differentiation, and survival. Polymorphism of NGFB gene leads to hereditary sensory and autonomic neuropathies. Charcot arthropathy and thermal insensitivity are few of the related symptoms. NGFβ is known to promote cancer cell proliferation and metastasis, also mediates angiogenesis. It facilitates the development of various cancer including, oral, breast, lungs, pancreatic and prostate cancers. Elevated expression of NGFβ contributes to progression of human cholangiocarcinoma. In animal models, NGFβ is observed to prevent cholinergic neuron loss and enhances memory. NGF-β promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

Forme physique

Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin.

Remarque sur l'analyse

The biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)