N3910

Neutralization Solution B

• Synonyme(s) : DNA neutralization solution, neutralization buffer DNA extraction
• Code UNSPSC : 12161703
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Forme: liquid
• Technique(s): DNA amplification: suitable; DNA extraction: suitable
• Température de stockage: −20°C

Description

Description générale

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp^™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Application

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp^™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp^™ PCR Reaction Mix.

Informations légales

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Pictogrammes: GHS07,GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H315,H317,H319,H411
• Conseils de prudence: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable