N7885

Neuraminidase from Vibrio cholerae

Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

• Synonyme(s) : Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
• CAS Number: 9001-67-6
• Numéro CE : 3.2.1.18 (BRENDA, IUBMB)
• Numéro CE : 232-624-6
• Numéro MDL: MFCD00131711
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Niveau de qualité: 300
• Type: Type III
• Forme: buffered aqueous solution
• Activité spécifique: 1-5 units/mg protein (Lowry, using NAN-lactose)
• Poids mol.: 83 kDa
• Application(s): diagnostic assay manufacturing
• Activité étrangère: Protease and NAN-aldolase, present
• Température de stockage: 2-8°C

Description

Description générale

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Actions biochimiques/physiologiques

Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells.

Qualité

Preservative free.

Définition de l'unité

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Forme physique

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl₂.

Notes préparatoires

Chromatographically purified.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)