N9914

Polynucleotide phosphorylase from Synechocystis sp.

recombinant, expressed in E. coli

• Synonyme(s) : PNPase, Polyribonucleotide Nucleotidyltransferase
• Numéro CE : 2.7.7.8 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131992
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bacterial (Synechocystis sp.)
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Description: Histidine tagged
• Essai: 90% (SDS-PAGE)
• Forme: solution
• Activité spécifique: ≥500 units/mg protein
• Poids mol.: 85 kDa
• Technique(s): cell based assay: suitable
• Adéquation: suitable for molecular biology
• Application(s): cell analysis
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase.

Application

Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor.

Actions biochimiques/physiologiques

Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity.

Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells.

Définition de l'unité

One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C.

Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl₂, 60 mM KCl, 20% (w/v) Glycerol

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable