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NXTRACT

Sigma-Aldrich

NuCLEAR™ Extraction Kit

For mammalian tissue or cultured cells

Synonyme(s) :

Nuclear Isolation Kit

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100 μL
₹58,993.00

₹58,993.00

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100 μL
₹58,993.00

About This Item

Code UNSPSC :
41105517
Nomenclature NACRES :
NA.56

₹58,993.00

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Niveau de qualité

300

Utilisation

 kit sufficient for 10 extractions (1 ml packed cell volume)
 kit sufficient for 100 extractions (100 μl packed cell volume)

Technique(s)

protein extraction: suitable
western blot: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Comparer avec des articles similaires

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1 of 4

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M-035

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Meperidine-D4 solution 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-036

Meperidine-D4 solution

Normeperidine-D4 solution 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

N-020

Normeperidine-D4 solution

application(s)

forensics and toxicology

application(s)

forensics and toxicology

application(s)

forensics and toxicology

application(s)

forensics and toxicology

technique(s)

gas chromatography (GC): suitable, liquid chromatography (LC): suitable

technique(s)

gas chromatography (GC): suitable, liquid chromatography (LC): suitable

technique(s)

gas chromatography (GC): suitable, liquid chromatography (LC): suitable

technique(s)

gas chromatography (GC): suitable, liquid chromatography (LC): suitable

form

liquid

form

liquid

form

liquid

form

liquid

format

single component solution

format

single component solution

format

single component solution

format

single component solution

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

grade

certified reference material

grade

certified reference material

grade

certified reference material

grade

certified reference material

Description générale

The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.

Application

NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice.[1] It was also used to test the therapeutic potential of andrographolide for treating endometriosis. [2]

Autres remarques

Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.

Liaison

Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549

Informations légales

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • 3× Dilution and Equilibration Buffer 90 mL
  • P8340Protease Inhibitor Cocktail 1 mLFDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H290,H314

Classification des risques

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

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    Certificats d'analyse (COA)

    Lot/Batch Number
    059M4108V
    11181117
    058M4141V
    11180227
    126M4144V

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    Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

    Consulter la Bibliothèque de documents

    Bettina Miller et al.
    Beilstein journal of organic chemistry, 8, 1091-1097 (2012-09-29)
    Hydrozirconation of 1-hexyne, the addition to in situ prepared N-acyliminium species, and ring-closing metathesis (RCM) were key steps in the preparation of a tricyclic isoindolinone scaffold. An unusual alkene isomerization process during the RCM was identified and studied in some
    Mauricio M Victor et al.
    Medicinal chemistry (Shariqah (United Arab Emirates)), 15(4), 400-408 (2018-10-27)
    The trypanosomatids, such as the protozoan Leishmania spp., have a demand by ergosterol, which is not present in the membrane from mammal cells. The suppression of the synthesis of ergosterol would be a new target of compounds with leishmanicidal activity
    David A Bichara et al.
    The Journal of arthroplasty, 33(8), 2666-2670 (2018-05-23)
    Wear resistance of ultrahigh molecular weight polyethylene (UHMWPE) is improved via ionizing radiation crosslinking and subsequent high temperature melting for improved toughness. Our group has previously reported that crosslinking can also be achieved chemically using organic peroxides. However, volatile peroxide
    Hyomin Jin et al.
    Molecules (Basel, Switzerland), 24(1) (2019-01-10)
    Herein, we investigated the effect of ring planarity by fully characterizing four pyridine-based o-carboranyl compounds. o-Carborane was introduced to the C4 position of the pyridine rings of 2-phenylpyridine and 2-(benzo[b]thiophen-2-yl)pyridine (CB1 and CB2, respectively), and the compounds were subsequently borylated
    He Nan et al.
    Journal of chromatography. A, 1523, 316-320 (2017-06-26)
    Silver ion or argentation chromatography utilizes stationary phases containing silver ions for the separation of unsaturated compounds. In this study, a mixed-ligand silver-based ionic liquid (IL) was evaluated for the first time as a gas chromatographic (GC) stationary phase for
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