O5500

Osmium tetroxide

Sealed ampule.

• Synonyme(s) : Osmium(VIII)-oxide, ‘Osmic acid’
• Formule linéaire : OsO₄
• CAS Number: 20816-12-0
• Poids moléculaire : 254.23
• Numéro CE : 244-058-7
• Numéro MDL: MFCD00011150
• Code UNSPSC : 12171500
• ID de substance PubChem : 57654490
• Nomenclature NACRES : NA.47

Propriétés

• Densité de vapeur: 8.8 (vs air)
• Niveau de qualité: 200
• Pression de vapeur: 7 mmHg ( 20 °C)
• Forme: powder
• Pertinence de la réaction: reagent type: oxidant
• pb: 130 °C (lit.)
• Pf: 39.5-41 °C (lit.)
• Chaîne SMILES: O=[Os](=O)(=O)=O
• InChI: 1S/4O.Os
• Clé InChI: VUVGYHUDAICLFK-UHFFFAOYSA-N

Description

Description générale

Osmium tetroxide (OsO4) is a heavy tetrahedral, symmetrical molecule composed of osmium, a third-row transition metal. Osmium tetroxide is both a dye, and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium testroxide to ensure preservation of the lipids.

Application

Osmium tetroxide has been used as a fixative reagent for Brevibacterium mcbrellneri, and curcumin treated human lung cancer cell lines′ observation with a scanning electron microscope. It is also suitable as a fixative for ultrastructural analysis of Toxoplasma gondii′s skeletal muscle cells.

Actions biochimiques/physiologiques

Osmium tetroxide chemically combines with lipids making them insoluble; only 7% of the lipids can be extracted after the fixation. Therefore, the cell membranes comprising phospholipids become black and electron-dense after fixation in osmium tetroxide. It is also used to fix small amount of fat maintained in sections during paraffin processing.

Autres remarques

Due to the hazardous nature of osmium tetroxide, it is recommended to use an ampule opener when using this chemical.

Informations sur la sécurité

• Pictogrammes: GHS06,GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H300 + H310 + H330,H314
• Conseils de prudence: P260 - P271 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Eye Dam. 1 - Skin Corr. 1B
• Code de la classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges