P4978

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

• Synonyme(s) : CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• CAS Number: 9001-78-9
• Numéro CE : 3.1.3.1 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131849
• Code UNSPSC : 12352204
• eCl@ss : 42010105
• Nomenclature NACRES : NA.53

Propriétés

• Qualité: Molecular Biology
• Forme: buffered aqueous glycerol solution
• Poids mol.: ~80 kDa
• Concentration: ≥10,000 units/mL
• Numéro d'accès UniProt: P19111
• Activité étrangère: DNase, RNase, none detected
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: cow ... ALPI(280993)

Description

Description générale

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Application

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Composants

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl₂, and 0.1 mM ZnCl₂, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Définition de l'unité

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Autres remarques

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable