P6374

p53 human

recombinant, expressed in baculovirus infected Sf21 cells

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in baculovirus infected Sf21 cells
• Essai: ≥90% (SDS-PAGE)
• Forme: aqueous solution
• Technique(s): dot blot: suitable
• Numéro d'accès UniProt: P04637
• Application(s): genomic analysis
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... TP53(7157)

Description

Description générale

p53 protein is composed of an N-terminal domain with a transactivation domain and proline-rich region, a central core-DNA binding domain, and a C-terminal domain with tetramerization and regulatory domains. The TP53 gene which encodes for the p53 protein is mapped on the human chromosome at 17p13.1.

Application

Useful for the study of postranslational modification of p53, or protein-protein interaction studies.

p53 human has been used in dot blot.

Actions biochimiques/physiologiques

In addition, p53 was recently shown to induce differentiation of human embryonic stem cells. The effect is dependent on the DNA binding activity of p53.

p53 gene is highly conserved and expressed in normal tissues. It is the most commonly mutated gene in human cancer and more then 500 gene mutations have been described in various types of malignancies, hematologic as well as solid tumors. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells. Thus, p53 plays a vital role in suppressing the development of cancer.

p53 protein acts as a transcription factor that participates in regulating positively or negatively the expression of several responsive genes. It plays a role in regulating angiogenesis, cellular senescence, autophagy, cell survival, differentiation, and oxidative stress. Mutations in the TP53 gene lead to Li Fraumeni cancer syndrome.

Propriétés physiques

Histidine-tagged, full length human p53

Forme physique

Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA, and 1 mM DTT.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable