P7275

Pyrophosphate reagent

spectrophotometric assay

• Synonyme(s) : Pyrophosphate detection reagent
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Niveau de qualité: 200
• Forme: powder
• Utilisation: vial sufficient for ≤4 assays
• Température de stockage: −20°C

Description

Application

The product has been used for the measurement of pyrophosphate content of plant tissues. It has also been used as a rapid, routine check of pyrophosphate content by monitoring NADH oxidation in Solanum tuberosum tuber extracts.

Actions biochimiques/physiologiques

The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH.

Conditionnement

A technical bulletin is included.

Composants

ComponentConcentration
Imidazole × HCl, pH 7.4 45 mM
Citrate 5 mM
EDTA 0.10 mM
Mg²⁺, Mn²⁺, Co²⁺ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
Bovine Serum Albumin 5 mg/mL
Sugar Stabilizer 5 mg/mL
PPi-PFK 0.5 units/mL
Aldolase 7.5 units/mL
GDH 5 units/mL
TPI 50 units/mL

Qualité

A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent), aldolase, triosephosphate isomerase, and α-glycerophosphate dehydrogenase.

Principe

Two moles of NADH are oxidized to NAD⁺ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm.
The reagent can be used to detect 10-100 nmoles of pyrophosphate.

Notes préparatoires

Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)