P7582

Amino-terminal FLAG-BAP^™ Fusion Protein

• Synonyme(s) : Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein
• Numéro MDL: MFCD00130921
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Forme: liquid
• Niveau de qualité: 200
• Poids mol.: ~49 kDa
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Amino-terminal FLAG-BAP^™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP^™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.

The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Application

Amino-terminal FLAG-BAP^™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.

Learn more product details in our FLAG^® application portal.

Autres remarques

Control protein

Forme physique

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Informations légales

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)