P8215

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use with fungal and yeast extracts, DMSO solution

• Synonyme(s) : Protease Inhibitor Mix, Protease inhibitor
• Numéro CE : 200-664-3
• Numéro MDL: MFCD00677817
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.54

Propriétés

• Product Name: Cocktail d′inhibiteurs de protéases, for use with fungal and yeast extracts, DMSO solution
• Niveau de qualité: 300
• Forme: DMSO solution
• Solubilité: water: soluble
• Température de stockage: −20°C

Description

Description générale

The Protease Inhibitor Cocktail for fungal and yeast extracts is a mixture of protease inhibitors in DMSO solution. The cocktail has been optimized and tested for use on fungal and yeast samples, specifically on Saccharomyces cerevisiae cells. One mL of the cocktail is recommended for the inhibition of proteases extracted from 20 g of yeast.

Spécificité

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

Protease Inhibitor Cocktail has been used as a component in:

• FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip)
• potassium phosphate buffer (pbs) to digest chitin
• extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis

Caractéristiques et avantages

• Broad specificity: inhibits a wide range of proteases, providing comprehensive protection to fungal and yeast extracts.
• Tested on Saccharomyces cerevisiae cells: the cocktail has been optimized for use on this commonly studied yeast strain.
• Convenient packaging: available in a 1 or 5 mL glass bottle for easy handling and storage.
• Ready-to-use solution: the cocktail is supplied in DMSO solution for immediate use in protease inhibition assays.
• Effective inhibition: each component in the cocktail has been carefully selected for its specific inhibitory properties, ensuring reliable and consistent results.

Composants

AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM

Autres remarques

Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Quantité

One mL is recommended for the inhibition of proteases extracted from 20 g of yeast.

Forme physique

Solution in DMSO

Informations sur la sécurité

• Pictogrammes: GHS07,GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H302,H315,H319,H410
• Conseils de prudence: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 188.6 °F
• Point d'éclair (°C): 87 °C