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PE0240

Sigma-Aldrich

Plant Fractionated Protein Extraction Kit

Suitable for any plant species or tissue

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About This Item

Code UNSPSC :
41105500
Nomenclature NACRES :
NA.56

Niveau de qualité

200

Utilisation

sufficient for 20 extractions

Température de stockage

−20°C

Description générale

Sigma′s Plant Fractionated Protein Extraction Kit is designed specifically for use in plant bioscience to extract qualitative hydrophilic and hydrophobic proteins samples from any type of plant species or tissue for downstream proteomic applications. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes five reagents; a plant specific protease inhibitor, a specially formulated reagent to extract hydrophilic proteins and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins. Also included in the kit are reducing reagent Tributylphosphine (TBP) and alkylating reagent, Iodoacetamide. These reagents improve separation during isoelectric focusing and 2-D gel electrophoresis. The protease inhibitor is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic, metalloproteases and aminopeptidases. The cocktail contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), bestatin, pepstatinA, E-64, leupeptin, and 1,10-phenanthroline.
Following removal of polyphenolics, tannins and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in a specially formulated reagent to extract hydrophilic proteins. After sequential extractions of hydrophilic proteins, addition of the chaotropic reagent provided extracts hydrophobic membrane bound proteins. Plant debris is pelleted by centrifugation and protein extract solutions are collected. The end results are qualitative fractionated protein samples, ready for downstream proteomic analysis.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • Plant Protein Extraction Reagent Type 1 1 bottle
  • C0356Protein Extraction Reagent Type 4 1 bottleFDS
  • P9599Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution 3 x 1FDS
  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 3 x 0.5FDS
  • A3221Iodoacetamide, Single use vial of 56 mg 3 vial(s)FDS

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Certificats d'analyse (COA)

Lot/Batch Number
SLCK1957

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