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PIC0006

Sigma-Aldrich

Protease Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, Non-freezing solution

Synonyme(s) :

Protease inhibitor cocktail

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.28

Product Name

ReadyShield® Protease Inhibitor Cocktail, for use in tissue culture media, Non-freezing solution

Description

Tissue Culture Media

Forme

ready-to-use solution

Qualité

200

Conditionnement

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

Solubilité

water: soluble

Température de stockage

−20°C

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Catégories apparentées

Description générale

This product is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. It is designed to be used as a supplement to tissue culture medium for the prevention of secreted proteins degradation. The product has been found to be non-toxic after a 48 hours exposure to the following adherent cell lines: A431, CHO, COS, HepG2, and HeLa, and to following non-adherent cell lines: Jurkat and HL-60. This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0006 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P1860.

Caractéristiques et avantages

  • Does not freeze in storage for quick, convenient use.
  • Inhibits serine, cysteine, aspartic proteases and aminopeptidases.
  • Tailored for use in tissue culture media.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Notes préparatoires

It is recommended to determine the appropriate dilution for each specific cell line. This testing should begin with a dilution of at least a 200-fold to prevent deleterious effect of the solvent to cell growth.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations légales

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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