R8508

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

buffered aqueous solution

• Synonyme(s) : Transfer RNA, tRNA
• CAS Number: 9014-25-9
• Numéro MDL: MFCD00132200
• Code UNSPSC : 12352106
• Nomenclature NACRES : NA.55

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Forme: buffered aqueous solution
• Concentration: 9-11 mg/mL
• Activité étrangère: DNase, Nickase, none detected
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Transfer RNA (tRNA) is isolated from baker′s yeast.

Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.

Application

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.

Actions biochimiques/physiologiques

Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.

Notes préparatoires

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)