RDRT

ReadyScript^® cDNA Synthesis Mix

Complete reagent for first strand cDNA synthesis for RT-qPCR

• Synonyme(s) : cDNA Synthesis Mix
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.55

Propriétés

• Utilisation: sufficient for 100 reactions; sufficient for 25 reactions; sufficient for 500 reactions
• Caractéristiques: dNTPs included; hotstart: no
• Technique(s): RT-PCR: suitable
• Couleur: colorless
• Entrée: purified RNA
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

ReadyScript^® cDNA Synthesis Mix is a sensitive and easy-to-use solution for two-step RT-PCR. This 5X concentrated mix provides all necessary components (except RNA template) for first-strand synthesis including: buffer, dNTPs, MgCl₂, primers, RNase inhibitor protein, ReadyScript reverse transcriptase and stabilizers. ReadyScript is a RNase H(+) derivative of MMLV reverse transcriptase, optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. The unique blend of oligo (dT) and random primers in the ReadyScript cDNA Synthesis Mix works exceptionally well with a wide variety of targets. It is optimized for the production of targets < 1kb in length. ReadyScript cDNA Synthesis Mix produces excellent results in both real-time and conventional RT-PCR.

Application

ReadyScript^® cDNA Synthesis Mix has been used for complementary DNA (cDNA) synthesis from total/mRNA in 2-step reverse transcription polymerase chain reaction (RT-PCR) and quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays for gene expression studies.

Caractéristiques et avantages

• Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
• Includes proprietary blend of randomers and oligodT primers and an RNAse H+ derivative of Moloney murine leukemia virus (MMLV) reverse transcriptase
• Allows for unbiased, accurate conversion of RNA to cDNA in just 40 minutes
• Can use up to 1 μg of RNA in a single 20 μL reaction; reactions can also be scaled up
• Highly stable; can be stored at -20°C for up to 1 year; 4°C for up to one month

• Specific ratio of randomers and oligo (dT) primers in the mastermix ensures optimal representation of all transcript sequences in the cDNA product
• High reproducibility: Premixed master mix eliminates variability inherent in other kits requiring mixing of components
• RNase H(+) Moloney murine leukemia virus (MMLV) reverse transcriptase ensures residual RNA does not interfere with PCR amplification
• Reverse transcribes both rare and abundant genes with a wide input range of template
• Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template

Composants

Supplied as a 5X reaction buffer containing optimized concentrations of MgCl₂, dNTPs (dATP, dCTP, dGTP, dTTP), recombinant RNase inhibitor protein, ReadyScript reverse transcriptase, random primers, oligo(dT) primer and stabilizers.

Autres remarques

Stable for 1 year when stored in a constant temperature freezer at -20 °C. To extend product′s shelf-life, store the kit at -70 °C. Readyscript cDNA Synthesis Mix showed no loss in functional performance after 20 cycles of freezing on dry ice and thawing on ice. However, we recommend keeping the freeze-thaw cycles to a minimum.

Informations légales

ReadyScript is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable