RECOMT

Thrombin CleanCleave^™ Kit

• Synonyme(s) : Thrombin Cleavage Kit
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.56

Propriétés

• Forme: suspension
• Niveau de qualité: 200
• Poids mol.: 37 kDa
• Technique(s): protein purification: suitable
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Application

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Caractéristiques et avantages

• Fast, efficient cleavage in as little as 2 hours
• Thrombin is covalently bound to agarose for easy removal.
• The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
• Cleave tags even in the presence of 0.1% Triton^™, 1 M urea or 5 mM EDTA
• Thrombin-agarose is reusable.

Quantité

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

Informations légales

CleanCleave is a trademark of Sigma-Aldrich Co. LLC

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Composants de kit seuls

• Thrombin Cleavage Buffer, 10× 10 mL

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable