S6147

Sephadex^® G-100

BioReagent, DNA grade, medium

• CAS Number: 9050-94-6
• Numéro MDL: MFCD00132235
• Code UNSPSC : 23151817
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Forme: beads
• Technique(s): buffer exchange: suitable
• Groupe de la matrice active: phase
• Gonflement: 1 g swells to 9-11 mL
• Diamètre des billes: 40-120 μm
• Dimension de pores: exclusion limit (25 bp dsDNA; (rA)27)
• pH: 2-10
• Application(s): life science and biopharma
• Compatibilité: Cytiva
• Activité étrangère: DNase, RNase, none detected
• Température de stockage: room temp

Description

Description générale

Sephadex^® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex^® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex^® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.

Caractéristiques et avantages

• Excellent recovery
• Minimal sample dilution
• Quick and easy to use

Informations légales

Sephadex is a registered trademark of Cytiva

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)