S7651

Sphingomyelinase from Bacillus cereus

lyophilized powder, ≥100 units/mg protein

• Synonyme(s) : Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
• CAS Number: 9031-54-3
• Numéro CE : 3.1.4.12 (BRENDA, IUBMB)
• Numéro CE : 232-869-9
• Numéro MDL: MFCD00132362
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Activité spécifique: ≥100 units/mg protein
• Poids mol.: 36873 Da
• Composition: Protein, ~10% Lowry
• Température de stockage: 2-8°C
• Informations sur le gène: Bacillus cereus ATCC 14579 ... BC0671(1203022)

Description

Description générale

Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg²⁺-containing metalloenzyme. Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.

Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages. Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.

Application

Sphingomyelinase from Bacillus cereus has been used:

• in the measurement of membrane capacitance in brain slices
• for testing and confirming its silkworm-killing activity
• in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease.

Actions biochimiques/physiologiques

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Forme physique

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317
• Conseils de prudence: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classification des risques: Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)