S9012

Sulforhodamine B sodium salt

Powder

• Synonyme(s) : Acid red 52; Acid Rhodamine B
• Formule empirique (notation de Hill) : C₂₇H₂₉N₂NaO₇S₂
• CAS Number: 3520-42-1
• Poids moléculaire : 580.65
• Numéro C.I. (Colour Index): 45100
• Beilstein: 3886008
• Numéro CE : 222-529-8
• Numéro MDL: MFCD00010180
• Code UNSPSC : 12171500
• ID de substance PubChem : 24899824
• Nomenclature NACRES : NA.47

Propriétés

• Product Name: Sulforhodamine B sodium salt, Technical grade
• Niveau de qualité: 200
• Forme: powder
• Composition: Dye content, ~75%
• εmax: ≥85000 at 554-560 nm
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1
• InChI: 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
• Clé InChI: SXQCTESRRZBPHJ-UHFFFAOYSA-M

Description

Description générale

Sulforhodamine B (also known as SRB, kiton rhodamine B, lissamine rhodamine B, or sulphorhodamine B) is a bright-pink anionic, aminoxanthene dye carrying two sulfonate substituents.

Application

Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.

It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.

It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)