SRP0356

GSK3β active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE)

• Synonyme(s) : glycogen synthase kinase 3 beta
• CAS Number: 443900-95-6
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Essai: ≥80% (SDS-PAGE)
• Forme: aqueous solution
• Activité spécifique: ≥500 pmol/min-μg
• Poids mol.: 73 kDa
• Conditionnement: pkg of 10 μg
• Technique(s): activity assay: suitable; inhibition assay: suitable
• Numéro d'accès NCBI: NM_002093
• Numéro d'accès UniProt: P49841
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... GSK3B(2932)

Description

Description générale

Glycogen Synthase Kinase 3β (GSK3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.

Human GSK3beta GenBank Accession No. NM_002093), full length with N-terminal GST-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple protein which are linked with Alzheimer′s disease (AD).

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable