SRP0719

ALK2 (R206H), Active

PRECISIO^® Kinase, recombinant

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Essai: 70-95% (SDS-PAGE)
• Forme: frozen liquid
• Activité spécifique: 5-7 nmol/min·mg (radiometric)
• Poids mol.: ~67 kDa
• Concentration: 0.1 μg/μL
• Numéro d'accès: NM_001105
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... ALK2(90)

Description

Application

Useful for kinase assay.

Actions biochimiques/physiologiques

Recombinant human ALK2 (R206H) (147-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. ALK2 belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins which encodes activin A type I receptor. ALK2 signals a particular transcriptional response in concert with activin type II receptors. Mutations in ALK2 are associated with fibrodysplasia ossificans progressiva (FOP) and the mutations are located in or adjacent to the GS regulatory region or active site of the kinase. The mutations in ALK2 were predicted by protein structure homology modeling to activate the ALK2 protein and enhance receptor signaling ⁽¹⁾. Dysregulated ALK2 kinase activity plays a role in the pathogenesis of FOP ⁽²⁾.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Stockage et stabilité

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Informations légales

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable