SRP8023

PARG human

recombinant, expressed in Sf21 cells, His tagged, >95% (SDS-PAGE)

• Synonyme(s) : FNDC5 (cleaved), Poly(ADP-ribose) glycohydrolase
• CAS Number: 9068-16-0
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in Sf21 cells
• Étiquette/Marqueur: His tagged
• Essai: >95% (SDS-PAGE)
• Forme: liquid
• Poids mol.: ~110 kDa by SDS-PAGE
• Conditionnement: pkg of 2 μg
• Concentration: ≥0.2 mg/mL
• Technique(s): cell based assay: suitable
• Couleur: clear
• Solubilité: water: soluble
• Numéro d'accès UniProt: Q86W56
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PARG(8505)

Description

Description générale

Research area: Cell signaling

Application

PARG human has been used in the digestion of sciatic nerve sections, in co-immunoprecipitation, in in vitro glycohydrolase activity assay

Actions biochimiques/physiologiques

Poly(ADP-ribose) glycohydrolase(PARG) hydrolyzes poly(ADP-ribose) at glycosidic(1′′-2′′) linkage of ribose-ribose bond to produce free ADP-ribose. It is an endo- and exo-glycosidase. PARG plays arole in DNA repair and replication. Inhibited or depleted PARG in cells aremore prone to DNA-damaging agents. PARG also averts the accumulation of PAR inthe cytoplasm and parthanatos, a caspase-independent PAR-mediated type of celldeath.

Forme physique

Solution in 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2% NP-40, 50 mM imidazole and 10% glycerol.

Autres remarques

Human PARG is fused to a His-tag.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H360D
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Repr. 1B
• Code de la classe de stockage: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable