T0256

Trypsin inhibitor from bovine pancreas

Type I-P, essentially salt-free, lyophilized powder

• Synonyme(s) : BPTI
• CAS Number: 9087-70-1
• Numéro CE : 232-906-9
• Numéro MDL: MFCD00082102
• Code UNSPSC : 12352202

Propriétés

• Source biologique: bovine pancreas
• Type: Type I-P
• Forme: essentially salt-free, lyophilized powder
• Poids mol.: ~6500 Da (single-chain 58-amino acid peptide)
• Produit purifié par: crystallization
• Technique(s): inhibition assay: suitable
• Solubilité: water: 10 g/L
• Température de stockage: −20°C

Description

Actions biochimiques/physiologiques

A 58-residue protein whose binding to various serine proteases has been extensively studied. It is highly homologous to a family of mamba snake dendrotoxin proteins that inhibit various K⁺ channels. It also binds to an intracellular site associated with the large conductance Ca²⁺-activated K⁺ channel..

Définition de l'unité

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Notes préparatoires

Prepared by method of Kunitz and Northrup, J. Gen. Physiol., 19, 991 (1936).

Remarque sur l'analyse

One mg of trypsin inhibitor will inhibit greater than 1.5 mg of trypsin with activity of approximately 10,000 BAEE units per mg protein.

Protein determined by biuret.

Autres remarques

View more information on Trypsin Inhibitor.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317,H334
• Conseils de prudence: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)