T1270

Thiamine hydrochloride

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

• Synonyme(s) : Aneurine hydrochloride, Vitamin B₁ hydrochloride
• Formule empirique (notation de Hill) : C₁₂H₁₇ClN₄OS · HCl
• CAS Number: 67-03-8
• Poids moléculaire : 337.27
• Beilstein: 3851771
• Numéro CE : 200-641-8
• Numéro MDL: MFCD00012780
• Code UNSPSC : 12352207
• eCl@ss : 34058006
• ID de substance PubChem : 24899952
• Nomenclature NACRES : NA.75

Propriétés

• Source biologique: synthetic (organic)
• Gamme de produits: BioReagent
• Essai: ≥99.0% (HPLC)
• Forme: powder
• Poids mol.: M_w 337.27 g/mol
• Technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable; cell culture | plant: suitable
• Couleur: white
• Pf: 250 °C (dec.) (lit.)
• Solubilité: H₂O: 50 mg/mL, clear, colorless
• Application(s): agriculture
• Chaîne SMILES: CC1=NC(N)=C(C[N+]2=CSC(CCO)=C2C)C=N1.Cl.[Cl-]
• InChI: 1S/C12H17N4OS.2ClH/c1-8-11(3-4-17)18-7-16(8)6-10-5-14-9(2)15-12(10)13;;/h5,7,17H,3-4,6H2,1-2H3,(H2,13,14,15);2*1H/q+1;;/p-1
• Clé InChI: DPJRMOMPQZCRJU-UHFFFAOYSA-M

Description

Description générale

Thiamine or Vitamin B1 is a positively charged and essential constituent for all tissues. It is present at higher levels in skeletal muscles, liver, kidney, heart, and brain. Thiamine is composed of a pyrimidine ring linked to a thiazole ring via a methylene bridge.

Application

Thiamine hydrochloride has been used as a component:

• of the vitamin-concentrated stock solution for the preparation of Hv-Ca medium for culturing Haloferax volcanii
• of the tris-acetate-phosphate (TAP) medium for culturing Chlamydomonas nivalis
• of modified Bold 3N medium for culturing Chlorella minutissima

Actions biochimiques/physiologiques

Thiamine is converted to thiamine diphosphate post-movement into the cellular membranes by thiamine diphosphokinase. Thiamine diphosphate, an active cofactor is essential for the mechanism of many enzymes involved in the citric acid cycle, the glycolytic pathway, the degradation of branched-chain amino acids, and the pentose phosphate pathway. Thiamine is involved in converting food into energy required for functioning the central and peripheral nervous systems. Lower levels of this vitamin are associated with delirium, Wernicke′s encephalopathy, and Wernicke-Korsakoff syndrome. Thiamine hydrochloride is required to support energy metabolism and amino acid synthesis in cultured cells. It is present in many classical and serum-free formulations.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H319
• Conseils de prudence: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Classification des risques: Eye Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)