T2807

Thymidine Phosphorylase, recombinant from Escherichia coli

recombinant, expressed in E. coli, buffered aqueous solution, ≥500 units/mL

• Synonyme(s) : Thymidine:orthophosphate deoxy-D-ribosyltransferase
• CAS Number: 9030-23-3
• Numéro CE : 2.4.2.4 (BRENDA, IUBMB)
• Numéro MDL: MFCD00132426
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 300
• Forme: buffered aqueous solution
• Concentration: ≥500 units/mL
• Numéro d'accès UniProt: P07650
• Température de stockage: 2-8°C
• Informations sur le gène: Escherichia coli K12 ... deoA(948901)

Description

Description générale

Thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.

Application

Thymidine phosphorylase has been used in a study to assess TAS-102 treatment in patients with advanced solid tumors. Thymidine phosphorylase has also been used in a study to investigate antitumor effects of the FP3 protein on patient-derived tumor tissue xenograft models of primary colon carcinoma.

Actions biochimiques/physiologiques

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway.

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

Définition de l'unité

One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

Forme physique

Solution in 0.5 M potassium phosphate containing 2 mM uracil, 0.02% sodium azide and bovine serum albumin

Notes préparatoires

Cloned from E. coli and produced in overexpressing E. coli

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)