T4528

Thioglucosidase from Sinapis alba (white mustard) seed

≥100 units/g solid

• Synonyme(s) : Glucosinolase, Thioglucoside glucohydrolase
• CAS Number: 9025-38-1
• Numéro CE : 3.2.1.147 (BRENDA, IUBMB)
• Numéro MDL: MFCD00132422
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: plant seeds (Sinapis alba)
• Niveau de qualité: 100
• Forme: solid
• Activité spécifique: ≥100 units/g solid
• Température de stockage: −20°C

Description

Description générale

Thioglucosidase, also called as myrosinase, is present in the myrosin cells that do not contain glucosinolates. This enzyme is obtained from several plant sources, such as Lepidium sativum, L. Sinapis alba and Brassica napus.

Application

Thioglucosidase from Sinapis alba (white mustard) seeds has been used as a standard to quantify myrosinase activity and in column glucosinolate analysis of plant samples.

Thioglucosidase has been used in a study to assess Brassica species screening for glucosinolate content. Thioglucosidase has also been used in a study to investigate a negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana.

Actions biochimiques/physiologiques

Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. The enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate primarily to the isothiocyanate, though thiocyanates and nitriles are also formed. Many of the isothiocyanate products of aliphatic and aromatic glucosinolates have cancer chemopreventive properties.

Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits.

Définition de l'unité

One unit will produce 1.0 μmole glucose per min from sinigrin at pH 6.0 at 25 °C.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)