T5816

Tricine

BioPerformance Certified, suitable for cell culture, ≥99% (titration)

• Synonyme(s) : N-[Tris(hydroxyméthyl)méthyl]glycine
• Formule linéaire : (HOCH₂)₃CNHCH₂CO₂H
• CAS Number: 5704-04-1
• Poids moléculaire : 179.17
• Beilstein: 1937804
• Numéro CE : 227-193-6
• Numéro MDL: MFCD00004277
• Code UNSPSC : 12161700
• ID de substance PubChem : 24900318
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 400
• Qualité: BioPerformance Certified
• Essai: ≥99% (titration)
• Forme: crystalline powder
• Conditions de stockage: dry at room temperature
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin and total aerobic microbial count, tested
• Couleur: white
• Plage de pH utile: 7.4-8.8
• pKa (25 °C): 8.1
• Pf: 187 °C
• Traces de cations: heavy metals (as Pb): ≤5 ppm
• Absorption: ≤0.02 at 290 at 20%
• Application(s): diagnostic assay manufacturing; general analytical; life science and biopharma; sample preparation
• Chaîne SMILES: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• Clé InChI: SEQKRHFRPICQDD-UHFFFAOYSA-N

Description

Description générale

Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography.

Application

Constituant de tampons destinés à la séparation de peptides de faible poids moléculaire.

Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radicals during the investigation of radiation-induced membrane damage. Additionally, it has played a role in isolating and purifying ribosomes engaged in the translation of specific mRNA and containing specific peptidyl-tRNA molecules. Tricine has also been employed in the synthesis of RNA oligonucleotides and the fabrication of high-density DNA microarrays, enabling the exploration of mechanisms underlying nucleic acid·ligand interactions and library preparation through photolithography.

Caractéristiques et avantages

• Suitable as a Buffer component, for Electrophoresis and Protein separation
• Effective Buffering from pH 7.4-8.8 (25 °C) with a pKa of 8.1 (25 °C)
• Tested for Endotoxins and Total Aerobic Microbial Count
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Autres remarques

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Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)