U4883

Urea

8 M (after reconstitution with 16 mL high purity water)

• Synonyme(s) : Urea solution, Carbamide, Carbonyldiamide
• Formule linéaire : NH₂CONH₂
• CAS Number: 57-13-6
• Poids moléculaire : 60.06
• Beilstein: 635724
• Numéro CE : 200-315-5
• Numéro MDL: MFCD00008022
• Code UNSPSC : 12352111
• ID de substance PubChem : 24900649
• Nomenclature NACRES : NA.25

Propriétés

• Forme: powder
• Niveau de qualité: 100
• Concentration: 8 M (after reconstitution with 16 mL high purity water)
• Solubilité: H₂O: soluble (1 vial plus 16 mL)
• Chaîne SMILES: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• Clé InChI: XSQUKJJJFZCRTK-UHFFFAOYSA-N

Description

Description générale

Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. It can also disturb hydrogen bonding present in DNA secondary structure.

Application

Useful for solubilizing and denaturing proteins in preparation for 2D-PAGE, enzymatic digests, IMAC Chromatography, and related procedures.
Urea has been used for protein sample preparation for reverse-phase nanospray liquid chromatography-tandem mass spectrometry.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)