U8501

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast

Type X, lyophilized powder, ≥40 units/mg protein

• Synonyme(s) : UTP:α-D-glucose-1-phosphate uridylyltransferase
• CAS Number: 9026-22-6
• Numéro CE : 2.7.7.9 (BRENDA, IUBMB)
• Numéro CE : 232-811-2
• Numéro MDL: MFCD00132561
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bakers yeast
• Niveau de qualité: 200
• Type: Type X
• Forme: lyophilized powder
• Activité spécifique: ≥40 units/mg protein
• Composition: Protein, 30-60% modified Warburg-Christian
• Activité étrangère: UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%; inorganic pyrophosphatase ≤0.5%
• Température de stockage: −20°C

Description

Description générale

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.

Application

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:

• in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-¹³C9
• to quantify Suc synthase (SUS) enzyme activity in rice
• to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes

Actions biochimiques/physiologiques

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg²⁺, Ca²⁺, Mn²⁺, Ni²⁺ for its activity.

Définition de l'unité

One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.

Forme physique

Lyophilized, sulfate-free powder containing citrate buffer salt

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)